Analysis of clinically relevant, diagnostic DNA by capillary zone and double-gradient gel slab electrophoresis
Identifieur interne : 003A98 ( Main/Exploration ); précédent : 003A97; suivant : 003A99Analysis of clinically relevant, diagnostic DNA by capillary zone and double-gradient gel slab electrophoresis
Auteurs : Pier Giorgio Righetti [Italie] ; Cecilia Gelfi [Italie]Source :
- Journal of Chromatography A [ 0021-9673 ] ; 1998.
English descriptors
- Teeft :
- Academic press, Allele, Amelogenin locus, Analytical biotechnology, Androgen, Androgen insensitivity syndrome, Androgen receptor, Androgen resistance, Antisense, Antisense oligonucleotides, Apolipoprotein, Background electrolyte, Boca raton, Broblast growth factor, Brosis, Capillary electrophoresis, Capillary electrophoretic separations, Capillary zone electrophoresis, Cftr gene, Chromatogr, Chromosome, Clinical diagnosis, Conformation polymorphism, Cremonesi, Deletion, Denaturant, Denaturing, Denaturing conditions, Denaturing gradient, Dgge, Different mutations, Diffuse bands, Double gradient, Dystrophin gene, Dystrophin gene exons, Electrophoresis, Ethidium bromide, Exon, Ferrari, Forensic medicine, Forensic medicine mitochondrial, Fragment, Gene, Genetic disorders, Genomic material, Heart disease, High sensitivity, Human cancer, Human genetics, Insensitivity, Linear polyacrylamide, Locus, Mccord, Methodology, Molecular defects, Muscular dystrophy, Mutant, Mutation, Nature genet, Normal individuals, Nucleic acids, Ohmic heat, Oligonucleotides, Peak ratio, Point mutants, Point mutations, Polio virus, Polyacrylamide, Polymer, Polymerase chain reaction, Polymorphic fragments, Polymorphism, Porosity gradient, Quantitative gene dosage, Refractory mutation system, Rflp, Righetti, Second gradient, Separation space, Sequence variations, Sieves detection references, Sieving, Simultaneous analysis, Single nucleotide substitutions, Small deletions, Sscp, Such fragments, Syndrome, Temperature gradient, Tgce, Tgce technique, Thermal gradient capillary electrophoresis, Variable number, Vntr, Vntrs, Voltage ramps, Whole series, Willebrand factor gene.
Abstract
Abstract: A number of applications of capillary zone electrophoresis (CZE) in sieving liquid polymers (notably linear polyacrylamides and cellulose) for the analysis of polymerase chain reaction products of clinically relevant, diagnostic DNA, are reviewed here. The fields covered are human genetics, quantitative gene dosage, microbiology and virology, forensic medicine and therapeutic DNA (notably antisense nucleotides). Some unique, novel developments are highlighted, such as (a) non-isocratic CZE, i.e., temperature-programmed CZE for detection of DNA point mutations and (b) the synthesis of novel N-substituted acrylamides, offering extreme resistance to alkaline hydrolysis, coupled with high hydrophilicity. In the field of denaturing gradient gel electrophoresis (DGGE), as routinely performed in gel slabs, a novel methodology is described, i.e., double-gradient DGGE. In this technique, two gradients are simultaneously applied along the migration direction; a chemical denaturing gradient, for partially unwinding homo- and hetero-duplexes of DNA and a porosity gradient, for re-compacting diffuse bands melting over a broader range of denaturing conditions. Both the CZE and the slab gel methodologies, with the latest developments described in this review, appear to be promising tools for screening diagnostic DNA.
Url:
DOI: 10.1016/S0021-9673(97)00304-X
Affiliations:
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gradient</term><term>Dgge</term><term>Different mutations</term><term>Diffuse bands</term><term>Double gradient</term><term>Dystrophin gene</term><term>Dystrophin gene exons</term><term>Electrophoresis</term><term>Ethidium bromide</term><term>Exon</term><term>Ferrari</term><term>Forensic medicine</term><term>Forensic medicine mitochondrial</term><term>Fragment</term><term>Gene</term><term>Genetic disorders</term><term>Genomic material</term><term>Heart disease</term><term>High sensitivity</term><term>Human cancer</term><term>Human genetics</term><term>Insensitivity</term><term>Linear polyacrylamide</term><term>Locus</term><term>Mccord</term><term>Methodology</term><term>Molecular defects</term><term>Muscular dystrophy</term><term>Mutant</term><term>Mutation</term><term>Nature genet</term><term>Normal individuals</term><term>Nucleic acids</term><term>Ohmic heat</term><term>Oligonucleotides</term><term>Peak ratio</term><term>Point mutants</term><term>Point mutations</term><term>Polio virus</term><term>Polyacrylamide</term><term>Polymer</term><term>Polymerase chain reaction</term><term>Polymorphic fragments</term><term>Polymorphism</term><term>Porosity gradient</term><term>Quantitative gene dosage</term><term>Refractory mutation system</term><term>Rflp</term><term>Righetti</term><term>Second gradient</term><term>Separation space</term><term>Sequence variations</term><term>Sieves detection references</term><term>Sieving</term><term>Simultaneous analysis</term><term>Single nucleotide substitutions</term><term>Small deletions</term><term>Sscp</term><term>Such fragments</term><term>Syndrome</term><term>Temperature gradient</term><term>Tgce</term><term>Tgce technique</term><term>Thermal gradient capillary electrophoresis</term><term>Variable number</term><term>Vntr</term><term>Vntrs</term><term>Voltage ramps</term><term>Whole series</term><term>Willebrand factor gene</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A number of applications of capillary zone electrophoresis (CZE) in sieving liquid polymers (notably linear polyacrylamides and cellulose) for the analysis of polymerase chain reaction products of clinically relevant, diagnostic DNA, are reviewed here. The fields covered are human genetics, quantitative gene dosage, microbiology and virology, forensic medicine and therapeutic DNA (notably antisense nucleotides). Some unique, novel developments are highlighted, such as (a) non-isocratic CZE, i.e., temperature-programmed CZE for detection of DNA point mutations and (b) the synthesis of novel N-substituted acrylamides, offering extreme resistance to alkaline hydrolysis, coupled with high hydrophilicity. In the field of denaturing gradient gel electrophoresis (DGGE), as routinely performed in gel slabs, a novel methodology is described, i.e., double-gradient DGGE. In this technique, two gradients are simultaneously applied along the migration direction; a chemical denaturing gradient, for partially unwinding homo- and hetero-duplexes of DNA and a porosity gradient, for re-compacting diffuse bands melting over a broader range of denaturing conditions. Both the CZE and the slab gel methodologies, with the latest developments described in this review, appear to be promising tools for screening diagnostic DNA.</div></front></TEI><affiliations><list><country><li>Italie</li></country><region><li>Lombardie</li></region><settlement><li>Milan</li></settlement></list><tree><country name="Italie"><noRegion><name sortKey="Righetti, Pier Giorgio" sort="Righetti, Pier Giorgio" uniqKey="Righetti P" first="Pier Giorgio" last="Righetti">Pier Giorgio Righetti</name></noRegion><name sortKey="Gelfi, Cecilia" sort="Gelfi, Cecilia" uniqKey="Gelfi C" first="Cecilia" last="Gelfi">Cecilia Gelfi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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